| 中文名称 | 去整合素样金属蛋白酶8抗体 |
| 别 名 | A Disintegrin And Metalloproteinase domain 8; A Disintegrin And Metalloproteinase domain 8; ADAM 8; ADAM 8 precursor; ADAM 8 precursor; ADAM metallopeptidase domain 8; ADAM8 protein; CD 156; CD156; CD156a; CD156a antigen; CD156a antigen; Cell surface antigen MS2; Cell surface antigen MS2; Human leukocyte differentiation antigen; ADAM8_HUMAN; Human leukocyte differentiation antigen; Macrophage cysteine rich glycoprotein; Macrophage cysteine rich glycoprotein; MGC134985; MS 2; MS2. |
| 研究领域 | 细胞生物 免疫学 信号转导 细胞凋亡 细胞骨架 细胞外基质 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Cow, ) |
| 产品应用 | ELISA=1:500-1000 Flow-Cyt=1μg/Test not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 87kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADAM8 52-91aa:51-150/824 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3. Function: Possible involvement in extravasation of leukocytes. Subunit: Interacts with FST3. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed on neutrophils and monocytes. Similarity: Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain. SWISS: P78325 Gene ID: 101 Database links: Entrez Gene: 101 Human Entrez Gene: 11501 Mouse Entrez Gene: 499285 Rat Omim: 602267 Human SwissProt: P78325 Human SwissProt: Q05910 Mouse Unigene: 501574 Human Unigene: 15969 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 细胞外基质蛋白 |
| 产品图片 |  Blank control: Mouse splenocytes(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).  Black line : Positive blank control A431); Negative blank control (HepG2）Green line : Primary Antibody (Rabbit Anti-ADAM8 antibody (bs-4195R) ) Orange line：Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488) A431（Positive）and HepG2（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM8 Antibody(bs-4195R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). Black line : Positive blank control A431); Negative blank control (HepG2）Green line : Primary Antibody (Rabbit Anti-ADAM8 antibody (bs-4195R) ) Orange line：Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488) A431（Positive）and HepG2（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM8 Antibody(bs-4195R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).  Blank control: A431.Primary Antibody (green line): Rabbit Anti-ADAM8 antibody (bs-4195R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |