TOM70 抗体Applications
| In-house applications |
ELISA, IF, WB, IHC, IP, FC Application not tested? Please see our Guarantee. |
| Published applications |
WB |
Publications |
6 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human |
|
Images
Prev12Next
 mouse liver tissue were subjected to SDS PAGE followed by western blot with 14528-1-AP(TOM70 antibody) at dilution of 1:1500 Immunohistochemical of paraffin-embedded human heart using 14528-1-AP(TOM70 antibody) at dilution of 1:50 (under 10x lens) Immunohistochemical of paraffin-embedded human heart using 14528-1-AP(TOM70 antibody) at dilution of 1:50 (under 40x lens) Immunofluorescent analysis of Hela cells, using TOMM70A antibody 14528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). IP Result of anti-TOM70 (IP:14528-1-AP, 3ug; Detection:14528-1-AP 1:500) with mouse brain tissue lysate 6000ug. 1X10^6 HeLa cells were stained with .2ug TOM70 antibody (14528-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.
|
| Positive WB detected in |
Mouse liver tissue, MCF7 cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue |
| Positive IP detected in |
Mouse brain tissue |
| Positive IHC detected in |
Human heart tissue, human kidney tissue, human liver tissue |
| Positive IF detected in |
Hela cells |
| Positive FC detected in |
HeLa cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:10-1:100 |
TOM70 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information TOM70 抗体
| Immunogen |
TOM70 fusion protein ag6027 |
Full name |
Translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae) |
Calculated molecular
weight |
67 kDa |
Observed molecular
weight |
67kd |
GenBank accession
number |
BC052994 |
Gene ID (NCBI) |
9868 |
| Gene symbol |
TOMM70A |
Synonyms |
TOM70, TOMM70A |
Background
|
TOMM70A (or TOM70) is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer. TOM70 抗体
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