YWHAE (phospho T232) Peptide
产品名称： YWHAE (phospho T232) Peptide
英文名称： YWHAE (phospho T232) Peptide
产品编号： P1255
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of YWHAE.
- Immunogen:
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T232 of human YWHAE.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Dot Blot (1:500)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Association of 14-3-3 epsilon gene haplotype with completed suicide in Japanese.
Yanagi M, Shirakawa O, Kitamura N, Okamura K, Sakurai K, Nishiguchi N, Hashimoto T, Nushida H, Ueno Y, Kanbe D, Kawamura M, Araki K, Nawa H, Maeda K.J Hum Genet. 2005;50(4):210-6. Epub 2005 Apr 19.
- 2.
- Phosphorylation of grb10 regulates its interaction with 14-3-3.
Urschel S, Bassermann F, Bai RY, Munch S, Peschel C, Duyster J.J Biol Chem. 2005 Apr 29;280(17):16987-93. Epub 2005 Feb 18.
- 3.
- Interactions between extracellular signal-regulated protein kinase 1, 14-3-3epsilon, and heat shock factor 1 during stress.
Wang X, Grammatikakis N, Siganou A, Stevenson MA, Calderwood SK.J Biol Chem. 2004 Nov 19;279(47):49460-9. Epub 2004 Sep 10.
- Applications
- Western Blot (Tissue lysate)
- The YWHAE (phospho T232) polyclonal antibody (Cat # PAB0395) is used in Western blot to detect YWHAE in mouse brain tissue lysate.
- Western Blot (Cell lysate)
- The YWHAE (phospho T232) polyclonal antibody (Cat # PAB0395) is used in Western blot to detect YWHAE in HL-60 cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with YWHAE (phospho T232) polyclonal antibody (Cat # PAB0395) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Entrez GeneID:
- 7531
- Protein Accession#:
- NP_006752;P62258
- Gene Name:
- YWHAE
- Gene Alias:
- 14-3-3E,FLJ45465,KCIP-1,MDCR,MDS
- Gene Description:
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq
- Other Designations:
- 14-3-3 epsilon,mitochondrial import stimulation factor L subunit,protein kinase C inhibitor protein-1,tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
- Gene Pathway
- Related Disease