Product: Anti-E-tag (GAPVPYPDPLEPR)-HRP pAb
Cat. No.: PC-313 (0.1 mg)
Procedure:
Rabbits were immunized with E-tag peptide
conjugated with KLH. After multiple
immunizations in Freund’s adjuvant, serum was
collected. Antibodies were immunoaffinitypurified
using the peptide immobilized on a solid
phase and conjugated with HRP.
Specification:
Direct ELISA: Antibody specificity was verified
against the peptide conjugated to BSA (E-tag-
BSA).
Host:
Rabbit
Format:
Liquid. 0.1 mL of HRP-conjugated polyclonal
antibody at 1.0 mg/mL in 50 mM HEPES pH 7.0,
0.1M sodium chloride with protein stabilizer and
0.01% thimerosal.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

ELISA: Use at a 1:5,000 – 1:15,000 dilution.

Western blot: Use at a 1:1,000 – 1:3,000
dilution.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.