MBP monoclonal antibody, clone 2H9
产品名称: MBP monoclonal antibody, clone 2H9
英文名称: MBP monoclonal antibody, clone 2H9
产品编号: MAB10804
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against partial recombinant MBP.
- Immunogen:
- Recombinant protein corresponding to human MBP.
- Host:
- Mouse
- Reactivity:
- Human
- Form:
- Liquid
- Isotype:
- IgG1
- Storage Buffer:
- In ascites (0.03% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot (Transfected lysate)
- Western blot analysis using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) against HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate.
- Western Blot (Recombinant protein)
- Western blot analysis using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) against human MBP (aa: 1-197) recombinant protein. (Expected MW is 47 kDa)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemical analysis of paraffin-embedded human brain (A) and human cerebellum (B) tissues using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) with DAB staining.
- Immunofluorescence
- Immunofluorescence analysis of MSCs cells using MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. MBP monoclonal antibody, clone 2H9 (Cat # MAB10804) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Entrez GeneID:
- 4155
- Gene Name:
- MBP
- Gene Alias:
- MGC99675
- Gene Description:
- myelin basic protein
- Omim ID:
- 159430
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq
- Other Designations:
- Golli-mbp,OTTHUMP00000174383,OTTHUMP00000174384,OTTHUMP00000174385,OTTHUMP00000174386
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